Development of a Tool for Marker-Assisted Selection in Perennial Ryegrass
This study presents the development of 363 primer pairs for SNP genotyping across the perennial ryegrass genome, enhancing marker-assisted selection efficiency and demonstrating high transferability to related forage grass species.
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Detail beschrijving
Perennial ryegrass breeding can benefit from molecular markers to accelerate genetic gains. This study designed 363 primer pairs targeting conserved regions flanking introns in elite genotypes, enabling genome-wide SNP discovery. An average of one SNP per 42 bases was found across seven genotypes. The primers showed 73–97% transferability in fescues and 77% in cocksfoot. Since only 38% of SNPs were heterozygous in multiple genotypes, genotype-specific SNP markers were developed to track allele segregation in progenies. This tool supports large-scale genotyping and precise marker-assisted selection in forage grass improvement programs.
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Details bijdrage
- Project
- Locatie
- France
- Auteurs
- Philippe BARRE, Gaëlle CHUPEAU, Vincent BÉGUIER, Pascal BOURDON, Sandrine FLAJOULOT, Catherine GALBRUN, Marie-Christine GRAS, Marie-Christine LE PASLIER, Dominique NOËL, Ludovic POINSARD, Wojciech PIETRASZEK, Michel ROMESTANT, Laurent SAINT-PIERRE, Jérôme SALSE, Jean-Paul SAMPOUX, Benoît THAREL et Dominique BRUNE
- Doel
- Adopt innovative practices
- Soort bestand
- document
- Gepubliceerd op
- 01 mei 2014
- Taal van herkomst
- French
- Officiële project website
- Innovations agronomiques
- Licensie
- CC BY