Combined Exudation and Rhizobiome Sampling from the Same Soil-Grown Plant
This document provides a step-by-step protocol for simultaneously sampling root exudates and rhizosphere soil from the same soil-grown plant to enhance throughput in medium-scale rhizosphere phenotyping.
Detail description
Problem
Sampling root exudates and rhizosphere soil from the same plant is highly challenging. Exudation sampling requires an intact root system that is still attached to the shoot while rhizosphere soil collection usually damages or destroys this plant material.
Solution
Using an exudate sampling method that involves gentle removal of all attached soil via washing reduces root damage. The method for rhizosphere soil collection as well as exudate collection are described below.
Benefits
The combined exudation and rhizobiome sampling from the same plant increases throughput and is therefore a recommended tool for medium-scale rhizosphere phenotyping.
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Contribution detail info
- Project
Root2Res
Root2Resilience: Root phenotyping and genetic improvement for rotational crops resilient to environmental change
- Location
- Europe
- Authors
- Eva Oburger, Carmen Escudero-Martinez
- Purpose
- Other
- File type
- document
- Created on
- Jul 30, 2026
- Origin language
- English
- Official project website
- Root2Res
- License
- CC BY