Detection and Quantification of Vairimorpha Microsporidia in Bees Using qPCR
Quantitative PCR method for simultaneous detection and quantification of Vairimorpha (Nosema) species in honey bees, bumble bees, and solitary bees
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Detail description
Detection of Vairimorpha (Nosema) apis, V. ceranae, and V. bombi in honey bees, bumble bees, and mason bees using a quantitative PCR method targeting the RPB1 gene. The method enables accurate quantification of microsporidia, supports co-infection analysis, and facilitates spillover studies between bee species. Validated in Apis mellifera, Bombus terrestris, and Osmia bicornis, it supports high-throughput screening and harmonised diagnostics.
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Contribution detail info
- Project
- Location
- France
- Authors
- Aurélie Babin, Frank Schurr, Marie-Pierre Rivière, Marie-Pierre Chauzat, Eric Dubois
- Purpose
- Other, Identify and secure funding opportunities
- File type
- document
- Created on
- Apr 12, 2026
- Origin language
- English
- Official project website
- PoshBee
- License
- CC BY
- Keywords