Enzymatic Hydrolysis of Thraustochytrid Biomass for n-3 PUFA and Bioactive Peptide Extraction

Enzymatic hydrolysis of Thraustochytrid biomass using Alcalase 2.4L enhances yield of n-3 PUFAs and bioactive peptides. This study, part of the ALG-AD project, evaluates hydrolysis efficiency under controlled conditions (50°C, 500 rpm, pH regulated with NaOH), focusing on P20D, P20F, and P20I strains. Degree of hydrolysis was measured via pH-stat method. Results show improved emulsifying properties and interfacial adsorption, with peptide profiles between 6 and 0.3 kDa. Freezing pre-treatment ruptures cells, releasing proteins and boosting hydrolysis. Endogenous enzymes remain inactive, eliminating need for pre-inactivation. Lipid and peptide analysis via SEC-HPLC confirms high DHA content and bioactive compound recovery. Findings support sustainable valorisation of microalgal biomass for nutraceutical and functional food applications.