Rice double haploid (DH) plants are produced mainly through anther culture. In order to improve the anther culture protocol, microspores of two japonica rice genotypes (NRVC980385 and H28) were subjected to three growth regulator combinations and four colchicine treatments on induction medium. In addition, a post anther culture procedure using colchicine or oryzalin was tested to induce double haploid plantlets from haploid plantlets. A cold pre-treatment of microspores for 9 days at 10 °C increased callus induction 50-fold in the NRCV980385 genotype. For both genotypes, 2 mg L−1 2,4-D and 1 mg L−1 kinetin on colchicine-free induction medium gave the best culture responses. The culturability of both genotypes changed on colchicine-supplemented induction media. A high genotype dependency was recorded for callus induction, callus regenerating green plantlets and regeneration of green double haploid plantlets. Colchicine at 300 mg L−1 for 48 h enhanced callus induction 100-fold in H28. Colchicine-supplemented media clearly improved green double haploid plantlet regeneration. We showed that the post-anther culture treatment of haploid plantlets at 500 mg L−1 of colchicine permitted fertile double haploid plantlets to be generated. Finally, an enhanced medium-throughput flow cytometry protocol for rice was tested to analyse all the plantlets from anther and post anther culture.
1/1
Agricultural run-off and subsurface drainage tiles transport a significant amount of nitrogen and phosphorus leached after fertilization. alchemia-nova GmbH in collaboration with University of Natural Resources and Life Sciences, Vienna developed two multi-layer vertical filter systems to address the agricultural run-off issue, which has been installed on the slope of an agricultural field in Mistelbach, Austria. While another multi-layer addressing subsurface drainage water is implemented in Gleisdorf, Austria. The goal is to develop a drainage filter system to retain water and nutrients. Both multi-layer filter systems contain biochar and other substrates with adsorption properties of nutrients (nitrogen, phosphorus). The filter system can be of practical use if an excess of nutrients being washed out is of concern in the fields of the practitioner by keeping the surrounding waters clean. This approach may result in economic value by re-using the saturated biochar as fertilizer and improving the soil structure, thus increasing long-term soil fertility. Link: https://wateragri.eu/a-bio-inspired-multilayer-drainage-system/
This project has received funding from the European Union’s Horizon 2020 research and innovation program under Grant Agreement No 858735This project has received funding from the European Union’s Horizon 2020 research and innovation program under Grant Agreement No 858735. FACTSHEET NANOCELLULOSE MEMBRANES FOR NUTRIENT RECOVERY Key information Functionalized nanocellulose membranes can take up nitrate and phosphate. These membranes can be put in a water treatment unit. As the membranes are biobased, degradable materials, they can after use be added to the soil, thus returning the leached nutrients back for their original purpose providing fertilizers (nutrient recycling).
Because variables such as temperature and humidity have a profound effect on the activity of crop pests, diseases and natural enemies, the ability to monitor environmental conditions within a crop has always been important for crop protection.