Biological control of chestnut blight using hypovirulence

Biological control of chestnut blight in Extremadura, Spain, using hypovirulence induced by CHV-1 virus infection in Cryphonectria parasitica. The CASTANEA operational group, led by the University of Santiago de Compostela under the FOREST4EU project, identifies local Vegetative Compatibility Groups (VCGs) and mating types (MAT) to select compatible hypovirulent strains. Molecular confirmation ensures strain compatibility. Inoculants are produced and applied via bark scratches to promote transmission. Field trials show promising results in reducing virulence and preserving chestnut plantations. The approach supports sustainable agroforestry by targeting fungal spread while maintaining genetic diversity.