Characterization of tomato leaf curl New Delhi virus infecting cucurbits in France

Tomato leaf curl New Delhi virus (ToLCNDV) is now emerging in the Mediterranean Basin, starting from Spain in 2012. It has been observed for the first time in France in 2020 in Gard and Bouches-du-Rhône. Mediterranean ToLCNDV is a bipartite begomovirus, causing leaf curl disease mainly in zucchini and others cucurbits, and genetically distinct from Asian (Indian)-ToLCNDV that has a broader host range. The cryptic species of whitefly Bemisia tabaci is the main insect vector of ToLCNDV while mechanical inoculation is also possible. Previous studies indicate that the Mediterranean clade represents a homogenous population, probably originating from a single introduction. We established a suitable protocol of inoculation and performed biological and molecular characterization of the French isolates in order to estimate their risks of emergence and their potential agronomic impact. Symptom observation of French ToLCNDV isolates on melon and zucchini showed two different types so-called “severe” and “recovery”. French ToLCNDV was found to be transmissible by Bemisia tabaci as expected but not by the greenhouse whitefly Trialeurodes vaporariorum. Host range analysis in experimental conditions suggest that Bryony and Tomato are susceptible to French and Spanish ToLCNDV and may constitute virus reservoirs, what should be taken into consideration for virus control. Our results expand the knowledge on this emerging virus and offer perspectives for shaping the future plant disease management.

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Detail příspěvku

Umístění
  • Europe
  • France
Autoři
  • Atiwich Patthamapornsirikul
  • Eric Verdin
  • Cecile Desbiez
Účel
  • Experimentation
  • Evaluation
  • Monitoring
  • Decision-making support
Typ souboru
Document
Velikost souboru
236 kB
Vytvořeno dne
05-05-2022
Jazyk původu
English
Oficiální webové stránky projektu
VIRTIGATION
Licence
CC BY

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